Capacitive imaging technique for non-destructive evaluation (NDE)

This thesis describes the development and characterization of a novel NDE methodthe Capacitive Imaging (CI) technique. The CI technique employs a pair of (or multiple) electrodes to form a co-planar capacitor, and uses the fringing quasi-static electric field established across the electrodes to investigate specimens of interest. In general, the CI probe is sensitive to surface and hidden defects in insulating materials, and surface features on conducting materials. The CI technique is advantageous for its non-contact and non-invasive nature, and the capacitive coupling allows the CI technique to work on a wide variety of material properties. The theoretical background to the CI technique has been developed. It is shown that in the frequency range of operation (10 kHz to 1 MHz), the quasi-static approximation is valid and the Maxwell’s Equations describing the general electromagnetic phenomena can be simplified. The practical implementation of the CI system is based on this analysis, and it is shown that the CI technique has features that can complement techniques such as eddy current methods that are already established in NDE. The design principles of the CI probes that are required for an optimum imaging performance have been determined, by considering the key measures of the performance including the depth of penetration, the measurement sensitivity, the imaging resolution and the signal to noise ratio (SNR). It has been shown that the operation frequency is not an influential factor - the performance of the CI probe is determined primarily by the geometry of the probe (e.g. size/shape of the electrodes, separation between electrodes, guard electrodes etc.). Symmetric CI probes with triangular-shaped electrodes were identified as a good general purpose design. Finite Element (FE) models were constructed both in 2D and 3D in COMSOLTM to predict the electric field distributions from CI probes. Effects of thickness of specimen, liftoff distance and relative permittivity value etc were examined using the 2D models. The sensitivity distributions of different CI probes were obtained from the 3D models and were used to characterize the imaging ability of the given CI probes. The fundamental concepts of the CI technique have been experimentally validated in a series of scans where the defects were successfully imaged in insulating (Perspex) and conducting (e.g. Aluminium, Steel and carbon fibre composite) specimens. The detection of corrosion under insulation (CUI) has also been demonstrated. The imaging abilities were assessed by investigating various standard specimens under different situations. The CI technique was then successfully applied to various practical specimens, including glass fibre laminated composites and sandwich structures, laminated carbon fibre composites, corroded steel plate and pipe, and concrete specimens. Further measurements were also conducted using modified CI probes, to demonstrate the wide range of applications of the CI technique

Non-destructive evaluation of concrete using a capacitive imaging technique : preliminary modelling and experiments

This paper describes the application of capacitive imaging to the inspection of concrete. A two-dimensional finite-element method was employed to model the electric field distribution from capacitive imaging probe, and how it interacts with concrete samples. Physical experiments with prototype capacitive imaging probes were also carried out. The proof-of-concept results indicated that the capacitive imaging technique could be used to detect cracks on the surface of concrete samples, as well as sub-surface air voids and steel reinforcement bars

Capacitive imaging technique for non-destructive evaluation (NDE)

This thesis describes the development and characterization of a novel NDE methodthe Capacitive Imaging (CI) technique. The CI technique employs a pair of (or multiple) electrodes to form a co-planar capacitor, and uses the fringing quasi-static electric field established across the electrodes to investigate specimens of interest. In general, the CI probe is sensitive to surface and hidden defects in insulating materials, and surface features on conducting materials. The CI technique is advantageous for its non-contact and non-invasive nature, and the capacitive coupling allows the CI technique to work on a wide variety of material properties. The theoretical background to the CI technique has been developed. It is shown that in the frequency range of operation (10 kHz to 1 MHz), the quasi-static approximation is valid and the Maxwell’s Equations describing the general electromagnetic phenomena can be simplified. The practical implementation of the CI system is based on this analysis, and it is shown that the CI technique has features that can complement techniques such as eddy current methods that are already established in NDE. The design principles of the CI probes that are required for an optimum imaging performance have been determined, by considering the key measures of the performance including the depth of penetration, the measurement sensitivity, the imaging resolution and the signal to noise ratio (SNR). It has been shown that the operation frequency is not an influential factor - the performance of the CI probe is determined primarily by the geometry of the probe (e.g. size/shape of the electrodes, separation between electrodes, guard electrodes etc.). Symmetric CI probes with triangular-shaped electrodes were identified as a good general purpose design. Finite Element (FE) models were constructed both in 2D and 3D in COMSOLTM to predict the electric field distributions from CI probes. Effects of thickness of specimen, liftoff distance and relative permittivity value etc were examined using the 2D models. The sensitivity distributions of different CI probes were obtained from the 3D models and were used to characterize the imaging ability of the given CI probes. The fundamental concepts of the CI technique have been experimentally validated in a series of scans where the defects were successfully imaged in insulating (Perspex) and conducting (e.g. Aluminium, Steel and carbon fibre composite) specimens. The detection of corrosion under insulation (CUI) has also been demonstrated. The imaging abilities were assessed by investigating various standard specimens under different situations. The CI technique was then successfully applied to various practical specimens, including glass fibre laminated composites and sandwich structures, laminated carbon fibre composites, corroded steel plate and pipe, and concrete specimens. Further measurements were also conducted using modified CI probes, to demonstrate the wide range of applications of the CI technique.EThOS - Electronic Theses Online ServiceUniversity of Warwick. School of EngineeringGBUnited Kingdo

Molecular estimation of alteration in intestinal microbial composition in Hashimoto's thyroiditis patients

The gut microbiota has a crucial effect on human health and physiology. Hypothyroid Hashimoto’s thyroiditis (HT) is an autoimmune disorder manifested with environmental and genetic factors. However, it is hypothesized that intestinal microbes might play a vital role in the pathogenesis of HT. The aim of current was to investigate and characterize the gut microbial composition of HT patients both quantitatively and qualitatively. The fecal samples from 29 HT patients and 12 healthy individuals were collected. The PCR-DGGE targeted V3 site of 16S rRNA gene and real time PCR for Bifidobacterium Lactobacillus, Bacteroides vulgatus and Clostridium leptum were performed. Pyrosequencing of 16S rRNA gene with V4 location was performed on 20 randomly selected samples. The comparative analysis of diversity and richness indices revealed diversification of gut microbiota in HT as compared to control. The statistical data elucidate the alterations in phyla of HT patients which was also affirmed at the family level. We observed the declined abundance of Prevotella_9 and Dialister, while elevated genera of the diseased group included Escherichia-Shigella and Parasutterella. The alteration in gut microbial configuration was also monitored at the species level, which showed an increased abundance of E. coli in HT. Therefore, the current study is in agreement with the hypothesis that HT patients have intestinal microbial dysbiosis. The taxa statistics at species-level along with each gut microbial community were modified in HT. Thus, the current study may offer the new insights into the treatment of HT patients, disease pathway, and mechanism

Relationships of beans intake with chronic kidney disease in rural adults: A large-scale cross-sectional study

Background and aimsDietary factors play an important role in the development of chronic kidney disease (CKD). However, evidence on the relationship of beans consumption with CKD remains limited and inconclusive, especially in the middle-and low-income populations. The current study aimed to investigate the relationships of beans intake with indicators of kidney injury and CKD prevalence in rural adults.MethodsA total of 20,733 rural adults from the Henan Rural Cohort Study in 2018–2022 were included. The total beans intake was collected using a validated food frequency questionnaire. Indicators of kidney injury and CKD was determined by the estimated glomerular filtration rate and the urinary albumin to creatinine ratio. Generalized linear regression and logistic regression models were applied to estimate the relationship of beans intake with continuous and dichotomized indicators of renal function, respectively.ResultsOf the 20,733 participants, 2,676 (12.91%) subjects were identified as CKD patients. After adjusting for potential confounders, participants in the higher quartiles of beans intake had a lower prevalence of CKD (odds ratio and 95% confidence interval, OR (95%CI); Q2: 0.968(0.866–1.082); Q3: 0.836(0.744–0.939); Q4: 0.854(0.751–0.970)) and albuminuria (Q2: 0.982(0.875–1.102); Q3: 0.846(0.750–0.954); Q4: 0.852 (0.746–0.973)), compared with the Q1. Per 50 g/day increment in beans intake was significantly associated with a 5 and 4% decreased prevalence of albuminuria and CKD, respectively. These inverse relationships were also significant in the subgroups of men, elder, and high-income participants (p < 0.05).ConclusionDietary beans intake was inversely associated with the prevalence of albuminuria and CKD in rural adults, suggesting that promoting soy food intake might help reduce the occurrence of CKD in rural adults

Molecular basis of ligand recognition and activation of human V2 vasopressin receptor.

Vasopressin type 2 receptor (V2R) belongs to the vasopressin (VP)/oxytocin (OT) receptor subfamily of G protein-coupled receptors (GPCRs), which comprises at least four closely related receptor subtypes: V1aR, V1bR, V2R, and OTR. These receptors are activated by arginine vasopressin (AVP) and OT, two endogenous nine-amino acid neurohypophysial hormones, which are thought to mediate a biologically conserved role in social behavior and sexual reproduction. V2R is mainly expressed in the renal collecting duct principal cells and mediates the antidiuretic action of AVP by accelerating water reabsorption, thereby playing a vital role in controlling water homeostasis. Moreover, numerous gain-of-function and loss-of-function mutations of V2R have been identified and are closely associated with human diseases, including nephrogenic syndrome of inappropriate diuresis (NSIAD) and X-linked congenital nephrogenic diabetes insipidus (NDI). Thus, V2R has attracted intense interest as a drug target. However, due to a lack of structural information, how AVP recognizes and activates V2R remains elusive, which hampers the V2R-targeted drug design. Here, we determined a 2.6 Å resolution cryo-EM structure of the full-length, G s -coupled human V2R bound to AVP (Fig. 1a; Supplementary information, Table S1). The G s protein was engineered based on mini-G s that was used in the crystal structure determination of the G s -coupled adenosine A 2A receptor (A 2A R) to stabilize the V2R–G s protein complex (Supplementary information, Data S1). The final structure of the AVP–V2R–G s complex contains all residues of AVP (residues 1–9), the Gα s Ras-like domain, Gβγ subunits, Nb35, scFv16, and the V2R residues from T31 to L339 8.57 (superscripts refer to Ballesteros–Weinstein numbering). The majority of amino acid side chains, including AVP, transmembrane domain (TMD), all flexible intracellular loops (ICLs) and extracellular loops (ECLs) except for ICL3 and G185–G188 in ECL2, were well resolved in the model, refined against the EM density map (Fig. 1a; Supplementary information, Figs. S1–3). The complex structure can provide detailed information on the binding interface between AVP and helix bundle of the receptor, as well as the receptor–G s interface

Solution-processed blue/deep blue and white phosphorescent organic light emitting diodes (PhOLEDs) hosted by a polysiloxane derivative with pendant mCP (1, 3-bis(9-carbazolyl)benzene)

The synthesis and characterization is reported of an efficient polysiloxane derivative containing the 1,3-bis(9-carbazolyl)benzene (mCP) moiety as a pendant unit on the polysiloxane backbone. In comparison with mCP, the mCP-polysiloxane hybrid (PmCPSi) has significantly improved thermal and morphological stabilities with a high decomposition temperature (Td = 523 °C) and glass transition temperature (Tg = 194 °C). The silicon–oxygen linkage of PmCPSi prevents intermolecular π-stacking and ensures a high triplet energy level (ET = 3.0 eV). Using PmCPSi as a host, blue phosphorescent organic light emitting devices (PhOLEDs) effectively confine triplet excitons, with efficient energy transfer to the guest emitter and a relatively low turn-on voltage of 5.8 V. A maximum external quantum efficiency of 9.24% and maximum current efficiency of 18.93 cd/A are obtained. These values are higher than for directly analogous poly(vinylcarbazole) (PVK) based devices (6.76%, 12.29 cd/A). Good color stability over a range of operating voltages is observed. A two-component “warm-white” device with a maximum current efficiency of 10.4 cd/A is obtained using a blend of blue and orange phosphorescent emitters as dopants in PmCPSi host. These results demonstrate that well-designed polysiloxane derivatives are highly efficient hosts suitable for low-cost solution-processed PhOLEDs

Non-destructive evaluation of composite materials using a capacitive imaging technique

This paper describes the application of the capacitive imaging technique to the inspection of composite materials. The fundamental theory of the capacitive imaging technique was briefly described. Experiments using prototype capacitive imaging probes were also carried out. The proof-of-concept results indicated that the capacitive imaging technique could be used to detect cracks and delaminations in the glass fibre composite, defects in the aluminium core through the glass fibre face as well as surface features on the carbon fibre specimens